Physical activity and amyloid beta in middle-aged and older adults: A systematic review and meta-analysis.

BACKGROUND: One of the pathological hallmarks distinguishing Alzheimer's disease from other dementias is the accumulation of amyloid beta (Aß). Higher physical activity is associated with decreased dementia risk, and one potential path could be through Aß levels modulation. We aimed to explore the relationship between physical activity and Aß in middle-aged and older adults. METHODS: A systematic search of PubMed, Web of Science, PsycINFO, Cochrane Central Register of Controlled Trials, and SPORTDiscus was performed from inception to April 28, 2022. Studies were eligible if they included physical activity and Aß data in adults aged 45 years or older. Multi-level meta-analyses of intervention and observational studies were performed to examine the role of physical activity in modulating Aß levels. RESULTS: In total, 37 articles were included (8 randomized controlled trials, 3 non-randomized controlled trials, 4 prospective longitudinal studies, and 22 cross-sectional studies). The overall effect size of physical activity interventions on changes in blood Aß was medium (pooled standardized mean difference = -0.69, 95% confidence interval (95%CI): -1.41 to 0.03; I2 = 74.6%). However, these results were not statistically significant, and there were not enough studies to explore the effects of physical activity on cerebrospinal fluid (CSF) and brain Aß. Data from observational studies were examined based on measurements of Aß in the brain using positron emission tomography scans, CSF, and blood. Higher physical activity was positively associated with Aß only in the CSF (Estimate r = 0.12; 95%CI: 0.05-0.18; I2 = 38.00%). CONCLUSION: Physical activity might moderately reduce blood Aß in middle-aged and older adults. However, results were only near statistical significance and might be interpreted with caution given the methodological limitations observed in some of the included studies. In observational studies, higher levels of physical activity were positively associated with Aß only in CSF. Therefore, further research is needed to understand the modulating role of physical activity in the brain, CSF, and blood Aß, as well as its implication for cognitive health.

Physical Performance and Amyloid-ß in Humans: A Systematic Review and Meta-Analysis of Observational Studies.

BACKGROUND: Accumulation of amyloid-ß (Aß) plaques is one of the main features of Alzheimer's disease (AD). Physical performance has been related to dementia risk and Aß, and it has been hypothesized as one of the mechanisms leading to greater accumulation of Aß. Yet, no evidence synthesis has been performed in humans. OBJECTIVE: To investigate the association of physical performance with Aß in humans, including Aß accumulation on brain, and Aß abnormalities measured in cerebrospinal fluid (CSF) and blood. METHODS: A systematic review with multilevel meta-analysis was performed from inception to June 16th, 2022. Studies were eligible if they examined the association of physical performance with Aß levels, including the measure of physical performance as a predictor and the measure of Aß as an outcome in humans. RESULTS: 7 articles including 2,619 participants were included in the meta-analysis. The results showed that physical performance was not associated with accumulation of Aß in the brain (ES = 0.01; 95% CI -0.21 to 0.24; I2 = 69.9%), in the CSF (ES = -0.28; 95% CI -0.98 to 0.41; I2 = 91.0%) or in the blood (ES = -0.19; 95% CI -0.61 to 0.24; I2 = 99.75%). Significant heterogeneity was found across the results , which posed challenges in arriving at consistent conclusions; and the limited number of studies hindered the opportunity to conduct a moderation analysis. CONCLUSIONS: The association between physical performance and Aß is inconclusive. This uncertainly arises from the limited number of studies, study design limitations, and heterogeneity of measurement approaches. More studies are needed to determine whether physical performance is related to Aß levels in humans.

Satisfacción con los cursos a distancia de la maestría Investigación en Aterosclerosis durante la COVID-19 / Satisfaction with the Distance Courses of a master's degree in Atherosclerosis Research during COVID-19

Introducción: La satisfacción de los estudiantes con respecto a la educación que reciben es un elemento clave en la valoración de la calidad de la educación. La enfermedad causada por el nuevo coronavirus SARS-CoV-2 representó una emergencia sanitaria que generó preocupación a nivel mundial por su impacto en la salud, la economía y la educación. Objetivo: Evaluar la satisfacción de los estudiantes con los cursos a distancia del primer ciclo de la maestría Investigación en Aterosclerosis durante la COVID-19. Métodos: Se hizo un estudio descriptivo de corte transversal. Se utilizó una encuesta, que estuvo disponible, al finalizar cada curso, en el Aula Virtual de Salud. Los datos de la encuesta se obtuvieron del AVS, se introdujeron en una base de datos en Excel, y se procesaron con el paquete estadístico SPSS versión 20.0 para Windows. Resultados: Para todos los cursos los horarios que predominaron fueron en horario extralaboral y los fines de semana y durante la jornada laboral, en horario extralaboral y los fines de semana. Como vías de conexión más utilizadas estuvieron Infomed y los datos móviles. El curso mejor orientado resultó La aterosclerosis, un problema epidemiológico. La evaluación de los aspectos relacionados con el profesor, los contenidos, el material bibliográfico, la comunicación y el aseguramiento tecnológico fue satisfactoria. La computadora y el celular representaron los dispositivos tecnológicos más empleados. Las respuestas en relación con la satisfacción con los cursos se encontraron entre satisfecho y muy satisfecho. Conclusiones: La mayor parte de los aspectos evaluados recibieron calificaciones satisfactorias. Como aspecto positivo se destaca el empleo del aula virtual; como negativos, la dificultad con los dispositivos tecnológicos y la conectividad; y como interesantes, la adquisición y el perfeccionamiento de habilidades con los cursos a distancia y mejorar la calidad de la conectividad(AU)

Introduction: Student satisfaction with the education that they receive is a key element for education quality assessment. The disease caused by the new SARS-CoV-2 coronavirus represented a health emergency that generated worldwide concern due to its impact on health, economy and education. Objective: To assess student satisfaction with the distance courses of the first cycle of a master's degree in atherosclerosis research during COVID-19. Methods: A descriptive and cross-sectional study was carried out. A survey was used, available in the virtual health classroom at the end of each course. The survey's data were obtained from the virtual health classroom, entered into an Excel database, and processed with the statistical package SPSS (version 20.0) for Windows. Results: For all the courses, the predominant schedules were during out-of-work hours and on weekends and during the working day, during out-of-work hours and on weekends. The most frequently used means of connection were Infomed and mobile data. The best oriented course was Atherosclerosis, an epidemiological problem. The assessment of aspects related to the professor, the contents, the bibliographic material, communication and technological assurance were satisfactory. The computer and the cell phone were the most frequently used technological devices. The responses regarding satisfaction with the courses ranged between satisfied and very satisfied. Conclusions: Most of the assessed aspects received satisfactory ratings. As a positive aspect, the use of the virtual classroom stands out; as a negative one, the difficulty with technological devices and connectivity; and as interesting ones, the acquisition and improvement of skills with distance courses, as well as improving the quality of connectivity(AU)

Satisfacción de los empleadores con el desempeño de egresados de la maestría "Investigación en aterosclerosis" / Employers' Satisfaction with the Performance of Graduates from a Master's Program about Research in Atherosclerosis

Introducción: Los estudios de empleadores cobran cada vez mayor importancia en el ámbito de la educación superior, a partir de la necesidad institucional de evaluar la pertinencia de sus programas e identificar expectativas de crecimiento, al permitir conocer la existencia de vinculación institucional y desarrollo de sus egresados. Objetivo: Describir la satisfacción de los empleadores con el desempeño de egresados de la décima edición de la maestría investigación en aterosclerosis. Métodos: Se hizo un estudio descriptivo de corte transversal. Se seleccionaron los empleadores de los egresados de la última edición terminada. Se les aplicó una encuesta de forma autoadministrada para explorar la satisfacción con el desempeño de los egresados de la maestría. Resultados: El total de los empleadores manifestaron que la maestría respondía a las necesidades asistenciales, docentes e investigativas del centro en el que laboraba el máster; que este aplicaba creativa y críticamente en su práctica diaria los conocimientos adquiridos en el programa de maestría; y que era capaz de diseñar y dirigir proyectos de investigación. El 94,1 por ciento respondió que, después de graduado de la maestría, el máster tenía la capacidad de diseñar y organizar cursos de superación para solucionar problemas de la institución. El 76,5 por ciento contestó que cumplía las expectativas y el 52,9 por ciento que se sentía satisfecho con el desempeño de los egresados. Conclusiones: Los empleadores manifestaron que los egresados satisfacían el perfil declarado en la maestría. Los egresados cumplieron con las expectativas de sus empleadores y los empleadores se encontraron satisfechos con el desempeño de los egresados(AU)

Introduction: Employer surveys are becoming increasingly important in the field of higher education, from the institutional need to evaluate the relevance of their programs and identify growth expectations, by allowing to know the existence of institutional linkage and the development of its graduates. Objective: To describe the satisfaction of employers with the performance of graduates from the tenth edition of a master's program about research in atherosclerosis. Methods: A descriptive and cross-sectional study was carried out. The employers of the graduates from the last completed edition were selected. A self-administered survey was conducted on them to explore satisfaction with the performance of the graduates from the master's program. Results: The whole number of employers stated that the master's degree responded to the care, teaching and research needs of the center where the master's degree holder worked; that he/she applied the knowledge acquired in the master's program creatively and critically in his/her daily practice; and hat he/she was capable of designing and directing research projects. 94.1 percent responded that, after graduating from the master's program, the master's degree holder had the capacity to design and organize professional improvement courses to solve the institutional problems. 76.5 percent answered that the master's degree met the expectations, while 52.9 percent responded that they were satisfied with the performance of the graduates. Conclusions: Employers stated that the graduates met the profile stated in the master's program. The graduates met the expectations of their employers, who were satisfied with the performance of the graduates(AU)

Observing a trained demonstrator influences associative appetitive learning in rats.

The ability to acquire information about the environment through social observation or instruction is an essential form of learning in humans and other animals. Here, we assessed the ability of rats to acquire an association between a light stimulus and the presentation of a reward that is either hidden (sucrose solution) or visible (food pellet) via observation of a trained demonstrator. Subsequent training of observers on the light-reward association indicated that while observation alone was not sufficient for observers to acquire the association, contact with the reward location was higher in observers that were paired with a demonstrator. However, this was only true when the light cue predicted a sucrose reward. Additionally, we found that in the visible reward condition, levels of demonstrator orienting and food cup contact during the observation period tended to be positively correlated with the corresponding behaviour of their observer. This relationship was only seen during later sessions of observer training. Together, these results suggest that while our models were not sufficient to induce associative learning through observation alone, demonstrator behaviour during observation did influence how their paired observer's behavioural response to the cue evolved over the course of direct individual training.

Bacteroides ovatus alleviates dysbiotic microbiota-induced intestinal graft-versus-host disease.

Acute gastrointestinal intestinal GVHD (aGI-GVHD) is a serious complication of allogeneic hematopoietic stem cell transplantation, and the intestinal microbiota is known to impact on its severity. However, an association between treatment response of aGI-GVHD and the intestinal microbiota has not been well-studied. In a cohort of patients with aGI-GVHD (n=37), we found that non-response to standard therapy with corticosteroids was associated with prior treatment with carbapenem antibiotics and loss of Bacteroides ovatus from the microbiome. In a mouse model of carbapenem-aggravated GVHD, introducing Bacteroides ovatus reduced severity of GVHD and improved survival. Bacteroides ovatus reduced degradation of colonic mucus by another intestinal commensal, Bacteroides thetaiotaomicron, via its ability to metabolize dietary polysaccharides into monosaccharides, which then inhibit mucus degradation by Bacteroides thetaiotaomicron and reduce GVHD-related mortality.

Mucus-degrading Bacteroides link carbapenems to aggravated graft-versus-host disease.

The intestinal microbiota is an important modulator of graft-versus-host disease (GVHD), which often complicates allogeneic hematopoietic stem cell transplantation (allo-HSCT). Broad-spectrum antibiotics such as carbapenems increase the risk for intestinal GVHD, but mechanisms are not well understood. In this study, we found that treatment with meropenem, a commonly used carbapenem, aggravates colonic GVHD in mice via the expansion of Bacteroides thetaiotaomicron (BT). BT has a broad ability to degrade dietary polysaccharides and host mucin glycans. BT in meropenem-treated allogeneic mice demonstrated upregulated expression of enzymes involved in the degradation of mucin glycans. These mice also had thinning of the colonic mucus layer and decreased levels of xylose in colonic luminal contents. Interestingly, oral xylose supplementation significantly prevented thinning of the colonic mucus layer in meropenem-treated mice. Specific nutritional supplementation strategies, including xylose supplementation, may combat antibiotic-mediated microbiome injury to reduce the risk for intestinal GVHD in allo-HSCT patients.

Dr. Netptali Taquechel Tusiente, paradigma de la historia y la medicina cubana / Dr. Netptali Taquechel Tusiente, a paradigm of Cuban history and medicine

La vida y obra de algunos hombres y mujeres de la historia de la Medicina, transita a veces, sin artilugios, acompañadas tan solo de la humildad y la sabiduría de estos. Es por ello, que al resumir en breves líneas el tiempo de enseñanzas y heroicidades de estos paradigmas, solo se cumple con el noble objetivo de exponer a las nuevas generaciones la labor realizada por maestros, como lo fue, el Dr. Netptali Taquechel Tusiente. A través de la realización de una revisión histórica de su vida y aportes y con la obtención de los datos por medio de la entrevista, el expediente laboral y académico como fuente primaria, se procesó la información. Los aportes de este internista estuvieron centrados en la especialidad de Medicina interna y en su actividad como líder del Ministerio de Salud Pública en diversos escenarios, pero el mayor de todos fue ser considerado una figura paradigmática de la salud pública. Se concluye que este médico cubano transitó de joven revolucionario y destacado estudiante a profesor de alto reconocimiento en la educación médica y figura inolvidable para todo el que lo conoció, con un merecido puesto entre los grandes de la historia de la Medicina cubana.

The life and work of some men and women in the history of medicine sometimes pass through without any gadgets, accompanied only by their humility and wisdom. That is why, when summarizing in few lines the time of teachings and heroisms of these paradigms, the noble objective of exposing to the new generations the work carried out by teachers such as Dr. Netptali Taquechel Tusiente is fulfilled. A historical review of the life and contributions of the aforementioned doctor was carried out, and the information was processed by obtaining data from interviews as well as from his employment and academic records as a primary source. The contributions of this internist were focused on the specialty of internal medicine as well as on his activity as a leader of the Ministry of Public Health in various settings, but the greatest of all was to be considered as a paradigmatic figure of public health. It is concluded that this Cuban doctor went from being a revolutionary young man and an outstanding student to a highly recognized professor in medical education and an unforgettable figure for all who knew him, with a deserved position among the greats in the history of Cuban medicine.

Encuestas para evaluar satisfacción con los cursos a distancia de la maestría Investigación en Aterosclerosis / Surveys for assessing satisfaction with the distance learning courses of the Master's Degree in Atherosclerosis Research

Introducción: La COVID-19 provocó que el Ministerio de Educación Superior indicara la suspensión de las actividades docentes de forma presencial. Por esta razón, la maestría Investigación en Aterosclerosis tuvo que pasar a la virtualidad. Objetivo: Validar el diseño de encuestas para la evaluación de la satisfacción de los maestrantes y profesores con los cursos virtuales de la maestría Investigación en Aterosclerosis. Métodos: Se realizó una investigación cualitativa que consistió en la revisión de la literatura publicada para analizar la teoría fundamentada en el diseño y la validación de encuesta. Se revisaron las publicaciones a partir de 2017 mediante el motor de búsqueda diseño y validación de instrumentos para evaluar la satisfacción con los cursos virtuales. También se hizo un análisis documental para analizar conceptos clave, ideas sobre los métodos utilizados para la elaboración de este tipo de instrumento; considerar problemáticas y retos de este diseño en otros estudios; y conocer las diferentes maneras de abordar el problema de investigación. Para el diseño de los instrumentos se llevó a cabo un estudio piloto con determinados estudiantes y profesores. Resultados: La encuesta para los estudiantes constó de 12 preguntas; y para los profesores, de 10, los cuales consideraron como satisfactoria su calidad. En el análisis de los resultados por los autores y la valoración global de ambas encuestas realizadas por el grupo de investigación surgieron dos recomendaciones para su perfeccionamiento, que se incluyeron para confeccionar la versión final de cada instrumento. Conclusiones: Ambas encuestas son fácilmente adaptables para utilizarse en otros cursos virtuales y en diferentes entornos virtuales de aprendizaje. Los dos instrumentos aportan datos generales, información sobre el profesor, contenido del curso, bibliografía y aseguramiento tecnológico(AU)

Introduction: COVID-19 caused the Ministry of Higher Education to indicate the interruption of face-to-face teaching activities. Subsequently, the master's degree in Atherosclerosis Research had to continue in the virtual modality. Objective: To validate survey design for assessing the satisfaction of master's degree students and professors with the virtual courses of the master's degree in Atherosclerosis Research. Methods: A qualitative research was conducted consisting of a review of the published literature to analyze the grounded theory in survey design and validation. Publications from 2017 were reviewed using the search, in which the following text chain was entered: design and validation of instruments to assess satisfaction with virtual courses. A documentary analysis was also conducted to analyze key concepts and ideas about the methods used for the development of this type of instrument, to consider problem situations and challenges of this design in other studies, as well as to learn about the different ways of approaching the research problem. For the design of the instruments, a pilot study was carried out with selected students and teachers. Results: The survey for the students consisted of twelve questions, while the professors had ten. The latter ones considered its quality as satisfactory. In the analysis of the results by authors and the overall assessment of both surveys carried out by the research group, two recommendations for improvement emerged, then included to make the final version of each instrument. Conclusions: Both surveys are easily adaptable for use in other virtual courses and in different virtual learning environments. Both instruments provide general data, information about the teacher, course content, bibliography and technological assurance(AU)

Relevance of Fluorinated Ligands to the Design of Metallodrugs for Their Potential Use in Cancer Treatment.

Fluorination of pharmaceutical agents has afforded crucial modifications to their pharmacological profiles, leading to important advances in medicinal chemistry. On the other hand, metallodrugs are considered to be valuable candidates in the treatment of several diseases, albeit with the caveat that they may exhibit pharmacological disadvantages, such as poor water solubility, low bioavailability and short circulating time. To surmount these limitations, two approaches have been developed: one based on the design of novel metallodrug-delivering carriers and the other based on optimizing the structure of the ligands bound to the metal center. In this context, fluorination of the ligands may bring beneficial changes (physicochemical and biological) that can help to elude the aforementioned drawbacks. Thus, in this review, we discuss the use of fluorinated ligands in the design of metallodrugs that may exhibit potential anticancer activity.

Improvement of Fish Growth and Metabolism by Oligosaccharide Prebiotic Supplement.

Finfish aquaculture is expected to continue to benefit from significantly improved fish diets, which are the source of energy to support the growth and health of fish. Strategies to enhance the transformation rate of dietary energy and protein to fish growth are greatly desired by fish culturists. Prebiotic compounds can be used as supplements to human, animal, and fish diets to populate beneficial bacteria in the gut. The goal of the present study is to identify low-cost prebiotic compounds with high efficacy in increasing the absorption of food nutrients by fish. Several oligosaccharides were evaluated as prebiotics in Nile tilapia (Oreochromis niloticus), one of the most widely cultured species in the world. Several parameters of the fish on different diets were evaluated, including feed conversion ratios (FCRs), enzymatic activities, expression of growth-related genes, and the gut microbiome. Two age groups of fish (30 days old and 90 days old) were used in this study. The results indicated that the addition of xylooligosaccharide (XOS), galactooligosaccharide (GOS), or XOS and GOS combination to the basic fish diet significantly decreased the feed conversion ratio (FCR) of the fish in both age groups. Both XOS and GOS decreased the FCR of 30-day-old fish by 34.4% compared to the fish on the control diet. In the 90-day-old fish group, XOS and GOS decreased the FCR by 11.9%, while the combination of the two prebiotics led to a 20.2% decrease in FCR compared to the control group. The application of XOS and GOS also elevated the production of glutathione-related enzymes and the enzymatic activity of glutathione peroxidase (GPX), indicating the enhancement of antioxidation processes in fish. These improvements were associated with significant changes in the fish gut microbiota. The abundance of Clostridium ruminantium, Brevinema andersonii, Shewanella amazonensis, Reyranella massiliensis, and Chitinilyticum aquatile were upregulated by XOS and GOS supplements. The findings of the present study suggested that the prebiotics would be more effective when they were applied to the younger fish, and the application of multiple oligosaccharide prebiotic compounds could result in a greater growth enhancement. The identified bacteria can be potentially used as probiotic supplements in the future to improve fish growth and feeding efficiency and ultimately reduce the cost of tilapia aquaculture.

Fluctuations in AKT and PTEN Activity Are Linked by the E3 Ubiquitin Ligase cCBL.

3-Poly-phosphoinositides (PIP3) regulate cell survival, division, and migration. Both PI3-kinase (phosphoinositide-3-kinase) and PTEN (phosphatase and tensin-homolog in chromosome 10) control PIP3 levels, but the mechanisms connecting PI3-kinase and PTEN are unknown. Using non-transformed cells, the activation kinetics of PTEN and of the PIP3-effector AKT were examined after the addition of growth factors. Both epidermal growth factor and serum induced the early activation of AKT and the simultaneous inactivation of PTEN (at ~5 min). This PIP3/AKT peak was followed by a general reduction in AKT activity coincident with the recovery of PTEN phosphatase activity (at ~10-15 min). Subsequent AKT peaks and troughs followed. The fluctuation in AKT activity was linked to that of PTEN; PTEN reconstitution in PTEN-null cells restored AKT fluctuations, while PTEN depletion in control cells abrogated them. The analysis of PTEN activity fluctuations after the addition of growth factors showed its inactivation at ~5 min to be simultaneous with its transient ubiquitination, which was regulated by the ubiquitin E3 ligase cCBL (casitas B-lineage lymphoma proto-oncogene). Protein-protein interaction analysis revealed cCBL to be brought into the proximity of PTEN in a PI3-kinase-dependent manner. These results reveal a mechanism for PI3-kinase/PTEN crosstalk and suggest that cCBL could be new target in strategies designed to modulate PTEN activity in cancer.

Manejo de la caries dental en tiempos de covid-19: Revisión de literatura / Management of dental caries in times of covid-19: A literature review

Antecedentes: En la odontología siempre se ha buscado la implementación de alternativas más conservadoras para el manejo de la caries dental, siendo en este caso una de las opciones el uso de Fluoruro Diamino de Plata más Yoduro de Potasio, el cual es un producto que de fácil aplicación que permite la inactivación de la Caries Dental.Objetivo:Determinar si es viable uso de Fluoruro Diamino de Plata más Yoduro de Potasio para la inactivación de lesiones cariosas. Materiales y Métodos:Estudio descriptivo cualitativo transversal para el cual se realizó una revisión de la literatura reciente asociada a revistas con bases de datos indexadas. Resultados:Se obtuvieron un total de 103 artículos, los cuales fueron analizados y seleccionados 40, siendo excluidos 63 por no cumplir los criterios de inclusión. Conclusión:El uso de Fluoruro Diaminode Plata más Yoduro de Potasio para la inactivación de lesiones cariosas, resulta ser una alternativa eficaz para evitar la emisión de partículas de aerosol, reduciendo en gran medida el riesgo de contagio del COVID-19 durante el tratamiento dental

Background: In dentistry, the implementation of more conservative alternatives for the management of dental caries has always been sought. One option is the use of Silver Diamine Fluoride plus Potassium Iodide, which is a product easy to apply and allows the inactivation of dental caries.Aim:To determine if it is feasible to use Silver Diamine Fluoride plus Potassium Iodide for the inactivation of carious lesions. Materials and methods:This is a retrospective cross-sectional documentary qualitative descriptive study for which a review of recent literature associated with journals with indexed databases was carried out. Results:A total of 103 articles were obtained, which were analyzed and 40 of the 103 were selected, for the reason that the remaining 63 did not meet the inclusion criteria. Conclusion:The use of Silver Diamine Fluoride plus Potassium Iodide for the inactivation of carious lesions, turns out to be an effective and viable alternative, that reduce the emission of aerosol particles, thus reducing the risk of infection of N-COVID-19 during dental treatment

Role of NRF2 in Lung Cancer.

The gene expression program induced by NRF2 transcription factor plays a critical role in cell defense responses against a broad variety of cellular stresses, most importantly oxidative stress. NRF2 stability is fine-tuned regulated by KEAP1, which drives its degradation in the absence of oxidative stress. In the context of cancer, NRF2 cytoprotective functions were initially linked to anti-oncogenic properties. However, in the last few decades, growing evidence indicates that NRF2 acts as a tumor driver, inducing metastasis and resistance to chemotherapy. Constitutive activation of NRF2 has been found to be frequent in several tumors, including some lung cancer sub-types and it has been associated to the maintenance of a malignant cell phenotype. This apparently contradictory effect of the NRF2/KEAP1 signaling pathway in cancer (cell protection against cancer versus pro-tumoral properties) has generated a great controversy about its functions in this disease. In this review, we will describe the molecular mechanism regulating this signaling pathway in physiological conditions and summarize the most important findings related to the role of NRF2/KEAP1 in lung cancer. The focus will be placed on NRF2 activation mechanisms, the implication of those in lung cancer progression and current therapeutic strategies directed at blocking NRF2 action.

Mechanochemistry: A Green Approach in the Preparation of Pharmaceutical Cocrystals.

Mechanochemistry is considered an alternative attractive greener approach to prepare diverse molecular compounds and has become an important synthetic tool in different fields (e.g., physics, chemistry, and material science) since is considered an ecofriendly procedure that can be carried out under solvent free conditions or in the presence of minimal quantities of solvent (catalytic amounts). Being able to substitute, in many cases, classical solution reactions often requiring significant amounts of solvents. These sustainable methods have had an enormous impact on a great variety of chemistry fields, including catalysis, organic synthesis, metal complexes formation, preparation of multicomponent pharmaceutical solid forms, etc. In this sense, we are interested in highlighting the advantages of mechanochemical methods on the obtaining of pharmaceutical cocrystals. Hence, in this review, we describe and discuss the relevance of mechanochemical procedures in the formation of multicomponent solid forms focusing on pharmaceutical cocrystals. Additionally, at the end of this paper, we collect a chronological survey of the most representative scientific papers reporting the mechanochemical synthesis of cocrystals.

Conocimientos y prácticas de odontología preventiva en odontólogos en México.

No disponible.

Intervención educativa para el mejoramiento humano desde la cultura en los estudiantes de ciencias médicas / Educational intervention for human improvement from culture in medical science students

RESUMEN Fundamento: es necesario fortalecer la cultura general en los educandos a través del vínculo de las artes, la historia y la medicina para la formación de un profesional integral y culto. Objetivo: elaborar un programa de intervención educativa desde la cultura general para el mejoramiento del comportamiento humano de los estudiantes de la carrera de Medicina. Métodos: se realizó una investigación de desarrollo en la Facultad de Ciencias Médicas "Julio Trigo López" en el primer semestre de 2019. Se utilizaron métodos teóricos: análisis-síntesis, inductivo-deductivo y sistémico estructural; y empíricos: revisión documental del plan de estudio D de la carrera de Medicina y el documento base para el diseño del plan de estudio E del Ministerio de Educación Superior, y un cuestionario de conocimientos a los alumnos antes y después de la aplicación del producto, el cual validado según postulados de Moriyama. Resultados: el análisis documental permitió establecer la ausencia de contenidos de cultura general vinculados a los programas de la carrera, mientras el cuestionario demostró inconsistencia en conocimientos asociados a elementos de la historia, el arte y la medicina, por lo que se elaboró un programa de intervención educativa para los estudiantes compuesto por cuatro temas y ocho actividades lectivas impartidas en cuatro conferencias y cuatro talleres. Conclusiones: fue valorada por criterios de expertos como adecuada. Una vez aplicada demostró su efectividad al ampliar o modificar los conocimientos de los alumnos sobre los temas impartidos.

ABSTRACT Background: it is necessary to strengthen the general culture in students through the link of arts, history and medicine for the formation of a comprehensive and cultured professional. Objective: to elaborate an educational intervention program from the general culture for the improvement of the human behavior of the students of the Medicine degree. Methods: a development investigation was carried out at "Julio Trigo López" Faculty of Medical Sciences in the first semester of 2019. Theoretical methods were used: analysis-synthesis, inductive-deductive and structural systemic; and empirical ones: documentary review of study plan D of the Medicine degree and the base document for the design of study plan E of the Ministry of Higher Education, and a knowledge questionnaire to students before and after the implementation of the product, validated according to Moriyama's postulates. Results: the documentary analysis allowed to establish the absence of contents of general culture linked to the programs of the degree, while the questionnaire showed inconsistency in knowledge associated with elements of history, art and medicine, so a program of educational intervention was elaborated for students consisting of four topics and eight teaching activities given in four conferences and four workshops. Conclusions: it was assessed by expert´s criteria as adequate. Once applied, it demonstrated its effectiveness by expanding or modifying students' knowledge of the subjects taught.

Perceived Devaluation and Discrimination toward mental illness Scale (PDDs): Its association with sociodemographic variables and interpersonal contact in a Mexican sample / Escala de Percepción de la Devaluación y Discriminación hacia la enfermedad mental (PDDs): su relación con variables sociodemográficas y el contacto interpersonal en una muestra mexicana

Abstract Introduction Research about stigma on mental illness in different sociocultural contexts is crucial to guide international efforts to reduce discrimination. Objective Analyze the psychometric properties of the Perceived Devaluation and Discrimination (PDD) scale adapted for the Mexican population and its relationship with sociodemographic variables and interpersonal contact. Method The study was based on a psychometric and cross-sectional design. The PDD scale was applied to a sample of 295 participants recruited through community programs in the south of Mexico City. Data were analyzed using confirmatory factor analysis to identify the subscales of PDDs. These subscales were compared by age, gender, and education using a univariate analysis. One-way analysis of variance (ANOVA) was then performed to determine whether there were differences in educational level between three PDDM-subscales. Results The PDD scale obtained an adequate global internal consistency (α = .78). Three PDDs factors were identified: myths and stereotypes, favorable attitudes, and social exclusion, which explain 57.8% of the total variance. The results shows an Χ2SB = 140.88 (df = 143, p = .05, CFI = 1, RMSEA = .000, CI [.000, .029]). Other findings showed significant differences in favorable attitudes toward mental illness subscale by gender and age. The ANOVA results for the social perception of the devaluation subscale reveal that those with a higher educational level could be more aware about devaluation in society toward people with mental illness than other groups with lower education (F = 4.88, p = .005). Discussion and conclusion The PDD-M scale adapted in Mexico is a culturally valid and reliable measure that could be useful for evaluating the variations and commonalities of public stigma in comparative studies between Latino populations and other international research contexts.

Resumen Introducción La investigación acerca del estigma de la enfermedad mental en distintos contextos sociales es fundamental para combatir la discriminación. Objetivo Analizar las propiedades psicométricas de la Escala de Percepción de la Devaluación y la Discriminación hacia las enfermedades mentales adaptada en México (PDD) y su relación con variables sociodemográficas y el contacto interpersonal. Método Se utilizó un diseño transversal ex post facto. Se aplicó la escala PDD-M en población adulta (n = 295) de la Ciudad de México. Los datos se analizaron mediante un análisis factorial confirmatorio y análisis multivariados. Resultados Se identifican tres áreas del PDD-M: mitos y estereotipos, actitudes favorables y percepción social de la devaluación y discriminación, que explican el 57.8% de la varianza. Se obtuvo un buen ajuste del modelo Χ2SB = 140.88 (df = 143, p = .05, CFI = 1, RMSEA = .000, CI [.000, .029]). La consistencia interna global de la prueba (α = .78) fue adecuada. Los resultados muestran diferencias significativas por género, edad y escolaridad. El análisis multivariado reveló que quienes tenían mayor nivel educativo eran más conscientes de la devaluación y la discriminación que quienes tenían escolaridades más bajas (F = 4.88, p = .005). Discusión y conclusión Se obtuvo una medición culturalmente válida y confiable del estigma público de la enfermedad mental (PDD-M) que puede servir para impulsar la investigación en esta línea a través de estudios comparativos en América Latina y en otros contextos internacionales.

Efectividad de las técnicas de manejo conductual en odontopediatría. Revisión sistemática / Eficácia das técnicas de gestão comportamental na odontologia pediátrica. Revisão sistemáticaArtigo da revisaoe-220163Vol 11 N° 1 Enero - Junio 2021 / Effectiveness of behavioral management techniques in pediatric dentistry. Systematic review

Objetivo: efectuar una revisión sistemática sobre la efectividad de las técnicas de manejo de la conducta en niños sistémicamente sanos, en la consulta de Odontopediatría. Métodos: Investigación descriptiva, retrospectiva, de estudios observacionales y ensayos clínicos aleatorios controlados que evaluaron la efectividad de las técnicas en niños y adolescentes de 03 a 18 años de edad. Resultados: criterios de inclusión artículos en inglés y español, publicados desde 2014 al 2019. El análisis de la calidad metodológica de los estudios se realizó mediante la guía Strobe y CASPe. Se identificaron términos de búsqueda en inglés en el tesauro MeSH en la base de datos Pub­M/Medline, Scopus /Elsevier, español, se utilizaron descriptores de las Ciencias de la Salud (DeCS/BIREME) en la base de datos SciELO.org Redalyc.org, Latindex, Dialnet y google académico. Hubo homogeneidad en los estudios respecto a la selección de escalas para medir ansiedad y miedo en los niños al recibir tratamiento preventivo, curativo o restaurador. Los estudios evaluaron mayoritariamente técnicas de distracción aplicadas con recursos audiovisuales para determinar efectividad sobre el comportamiento de los niños en el consultorio odontológico. También reportaron disminución de la ansiedad y miedo, después de la aplicación de técnicas de distracción, e informaron que hubo cambios de conducta de pacientes ansiosos y negativos a pacientes positivos y colaboradores. Conclusiones: estudios mostraron una insuficiente calidad en sus diseños, lo que no permitió emitir juicios respecto a la contundencia y solidez de la evidencia sobre la efectividad de las técnicas aplicadas.

Objetivo: Realizar uma revisão sistemática da eficácia das técnicas de gerenciamento de comportamento em crianças sistemicamente saudáveis, na consultório de Odontopediatria. Métodos: Estudos descritivos, retrospectivos, observacionais e ensaios clínicos controlados e randomizados que avaliaram a eficácia das técnicas em crianças e adolescentes de 03 a 18 anos. Resultados: Os critérios de inclusão foram artigos em inglês e espanhol, publicados de 2014 a 2019. A análise da qualidade metodológica dos estudos foi realizada na guia Strobe and Consort. Os termos de pesquisa em inglês foram identificados na tesauro MeSH na base de dados PubMed / Medline, Scopus / Elsevier e, em espanhol, os descritores de Ciências da Saúde (DeCS / BIREME) foram utilizados na base de dados SciELO.org Redalyc.org, Latindex, Dialnet e Google Scholar. Houve homogeneidade nos estudos em relação à seleção de escalas para medir ansiedade e medo em crianças ao receber tratamento preventivo, curativo ou restaurador. Os estudos avaliaram principalmente técnicas de distração aplicadas com recursos audiovisuais para determinar sua eficácia na comportamento de crianças na consultório odontológico. Eles também relataram diminuição da ansiedade e medo, após a aplicação de técnicas de distração, e relataram que houve mudanças comportamentais de pacientes ansiosos e negativos para pacientes positivos e colaborativos. Conclusões: Os estudos mostraram qualidade insuficiente em seus desenhos, o que não permitiu julgamentos sobre a força e a força das evidências sobre a eficácia das técnicas aplicadas.

Aim: to conduct a systematic review of the effectiveness of behavior management techniques in systemically healthy children, in the Pediatric Dentistry office. Methods: Descriptive, retrospective, observational studies and randomized controlled clinical trials that evaluated the effectiveness of the techniques in children and teenangers aged 03 to 18 years. Results: The inclusion criteria were articles in English and Spanish, published from 2014 to 2019. The analysis of the methodological quality of the studies was carried out using the Strobe and Consort guide. English search terms were identified in the MeSH thesaurus in the Pub¬M / Medline, Scopus / Elsevier database, and in Spanish, descriptors of Health Sciences (DeCS / BIREME) were used in the SciELO.org database, Redalyc.org, Latindex, Dialnet and Google Scholar. There was homogeneity in the studies regarding the selection of scales to measure anxiety and fear in children when receiving preventive, curative or restorative treatment. The studies mostly evaluated distraction techniques applied with audiovisual resources to determine their effectiveness on the behavior of children in the dentistry office. They also reported decreased anxiety and fear, after the application of distraction techniques, and reported that there were behavioral changes from anxious and negative patients to positive and collaborative patients. Conclusions: The studies showed insufficient quality in their designs, which did not allow judgments regarding the strength and strength of the evidence on the effectiveness of the techniques applied.